Prof. Dr. Bärbel Friedrich
Research interests
Hydrogenase characterisation, physiology and biochemistry
Research Fields in UniCat
2012: Research Field E3 and Research Field E4
2007-2011: B2, C2
Selection of research projects
Relevant News
Tuesday, 13. December 2016
Leopoldina Medal of Merit for Bärbel Friedrich
Today, microbiologist Prof. Bärbel Friedrich and former member of UniCat has been awarded the Medal of Merit of the German National Academy of Sciences Leopoldina.
Monday, 05. December 2016
Research Highlight "Biosynthesis of the metal carbonyl in the catalytic center of [NiFe]-hydrogenase"
Activation of dihydrogen is by far not a trivial catalytic reaction. Microbes have evolved sophisticated hydrogenases with complex transition metal centers to get access to dihydrogen (H2). A recurring feature of these centers is the presence of iron atoms[more]
Wednesday, 09. April 2014
Reversible [4Fe-3S] cluster morphing in an oxygen-tolerant [NiFe] hydrogenase
New article by seven UniCat groups in Nature Chemical Biology[more]
Tuesday, 12. March 2013
Federal Cross of Merit for UniCat Professor Bärbel Friedrich
The microbiologist, Prof. Dr. Bärbel Friedrich was honoured with the Merit Cross on Ribbon by President Joachim Gauck on 7 March 2013 at the Bellevue Palace in Berlin for her contributions to the sciences in the new federal States. [more]
Tuesday, 21. February 2012
New Iron–Sulfur Clusters Help Hydrogenases Tolerate Oxygen
Nature publication of Oliver Lenz and Bärbel Friedrich has been highlighted in Angwandte Chemie Internationl Edition[more]
Friday, 21. October 2011
Structure of a microbial hydrogen engine
New results on biological hydrogen conversion published in Nature by UniCat researchers Oliver Lenz and Bärbel Friedrich[more]
UniCat publications
| 2018 CO synthesized from the central one-carbon pool as source for the iron carbonyl in O2-tolerant [NiFe]-hydrogenase I. Bürstel, E. Siebert, S. Frielingsdorf, I. Zebger, B. Friedrich, O. Lenz Proceedings of the National Academy of Sciences 2016, 113, 14722–14726, 10.1073/pnas.1614656113 |
| 1684 Cofactor composition and function of a H2-sensing regulatory hydrogenase as revealed by Mössbauer and EPR Spectroscopy F. Roncaroli, E. Bill, B. Friedrich, O. Lenz, W. Lubitz, M. E. Pandelia Chem. Sci. 2015, 10.1039/C5SC01560J |
| 1641 Oxygen-tolerant hydrogenases and their biotechnological potential O. Lenz, L. Lauterbach, S. Frielingsdorf, B. Friedrich Biohydrogen Matthias Rögner De Gruyter 2015, |
| 1414 Rubredoxin-related maturation factor guarantees metal cofactor integrity during aerobic biosynthesis of membrane-bound [NiFe] hydrogenase J. Fritsch, E. Siebert, J. Priebe, I. Zebger, F. Lendzian, C. Teutloff, B. Friedrich, O. Lenz Journal of Biological Chemistry 2014, 289, 7982–7993, 10.1074/jbc.M113.544668 |
| 1396 Reversible [4Fe-3S] cluster morphing in an O2-tolerant [NiFe] hydrogenase S. Frielingsdorf, J. Fritsch, A. Schmidt, M. Hammer, J. Löwenstein, E. Siebert, V. Pelmenschikov, T. Jaenicke, J. Kalms, Y. Rippers, F. Lendzian, I. Zebger, C. Teutloff, M. Kaupp, R. Bittl, P. Hildebrandt, B. Friedrich, O. Lenz, P. Scheerer Nature Chemical Biology 2014, 10, 378–385, 10.1038/nchembio.1500 |
| 1227 Protein-Protein Complex Formation Affects the Ni-Fe and Fe-S Centers in the H2-Sensing Regulatory Hydrogenase from Ralstonia eutropha H16 S. Löscher, A. Gebler, M. Stein, O. Sanganas, T. Buhrke, I. Zebger, H. Dau, B. Friedrich, O. Lenz, M. Haumann ChemSusChem 2010, 11, 1297–1306, 10.1002/cphc.200901007 |
| 1162 Novel, Oxygen-Insensitive Group 5 [NiFe]-Hydrogenase in Ralstonia eutropha C. Schafer, B. Friedrich, O. Lenz Applied and Environmental Microbiology 2013, 79, 5137–5145, 10.1128/AEM.01576-13 |
| 1041 Structure, function and biosynthesis of O2-tolerant hydrogenases J. Fritsch, O. Lenz, B. Friedrich Nature Reviews Microbiology 2013, 11, 106–114, 10.1038/nrmicro2940 |
| 872 The crystal structure of an oxygen-tolerant hydrogenase uncovers a novel iron-sulphur centre. J. Fritsch, P. Scheerer, S. Frielingsdorf, S. Kroschinsky, B. Friedrich, O. Lenz, C. M. T. Spahn Nature 2011, 479, 249–252, 10.1038/nature10505 |
| 869 A Universal Scaffold for Synthesis of the Fe(CN)(2)(CO) Moiety of [NiFe] Hydrogenase I. Buerstel, E. Siebert, G. Winter, P. Hummel, I. Zebger, B. Friedrich, O. Lenz Journal of Biological Chemistry 2012, 287, 38845–38853, 10.1074/jbc.M112.376947 |
| 780 Probing the origin of the metabolic precursor of the CO ligand in the catalytic center of [NiFe]-hydrogenase I. Bürstel, P. Hummel, E. Siebert, N. Wisitruangsakul, I. Zebger, B. Friedrich, O. Lenz Journal of Biological Chemistry 2011, 286, 44937–44944, 10.1074/jbc.M111.309351 |
| 775 A Trimeric Supercomplex of the Oxygen-Tolerant Membrane-Bound [NiFe]-Hydrogenase from Ralstonia eutropha H16 S. Frielingsdorf, T. Schubert, A. Pohlmann, O. Lenz, B. Friedrich Biochemistry 2011, 50, 10836–10843, 10.1021/bi201594m |
| 772 Role of the HoxZ Subunit in the Electron Transfer Pathway of the Membrane-Bound [NiFe]-Hydrogenase from Ralstonia eutropha Immobilized on Electrodes M. Sezer, S. Frielingsdorf, D. Millo, I. Zebger, T. Utesch, M. A. Mroginski, N. Heidary, B. Friedrich, P. Hildebrandt, I. Weidinger Journal of Physical Chemistry B 2011, 115, 10368–10374, 10.1021/jp204665r |
| 762 Oxygen-Tolerant [NiFe]-Hydrogenases: The Individual and Collective Importance of Supernumerary Cysteines at the Proximal Fe-S Cluster M. J. Lukey, M. M. Roessler, A. Parkin, R. M. Evans, R. A. Davies, O. Lenz, B. Friedrich, F. Sargent, F. A. Armstrong Journal of the American Chemical Society 2011, 133, 16881–16892, 10.1021/ja205393w |
| 760 [NiFe] and [FeS] Cofactors in the Membrane-Bound Hydrogenase of Ralstonia eutropha Investigated by X-ray Absorption Spectroscopy: Insights into O2-Tolerant H2 Cleavage F. Johannes, S. Löscher, O. Sanganas, E. Siebert, I. Zebger, M. Stein, M. Ludwig, A. L. De Lacey, H. Dau, B. Friedrich, O. Lenz, M. Haumann Biochemistry 2011, 50, 5858–5869, 10.1021/bi200367u |
| 578 The maturation factors HoxR and HoxT contribute to oxygen tolerance of membrane-bound [NiFe] hydrogenase in Ralstonia eutropha H16 J. Fritsch, O. Lenz, B. Friedrich Journal of Bacteriology 2011, 193, 2487–2497, 10.1128/JB.01427-10 |
| 577 A unique iron-sulfur cluster is crucial for oxygen tolerance of a [NiFe]-hydrogenase T. Goris, A. F. Wait, M. Saggu, J. Fritsch, N. Heidary, M. Stein, I. Zebger, F. Lendzian, F. A. Armstrong, B. Friedrich, O. Lenz Nature Chemical Biology 2011, 7, 310–318, 10.1038/NCHEMBIO.555 |
| 456 Oxygen-tolerant hydrogenases in hydrogen-based technologies B. Friedrich, J. Fritsch, O. Lenz Current Opinion in Biotechnology 2011, 22, 358–364, 10.1016/j.copbio.2011.01.006 |
| 434 Gas pressure effects on the rates of catalytic H2 oxidation by hydrogenases J. Cracknell, B. Friedrich, F. Armstrong Chemical Communications 2010, 46, 8463–8465, 10.1039/C0CC03292A |
| 355 H2 conversion in the presence of O2 as performed by the membrane-bound [NiFe]-hydrogenase of Ralstonia eutropha O. Lenz, M. Ludwig, T. Schubert, I. Buerstel, S. Ganskow, T. Goris, A. Schwarze, B. Friedrich ChemPhysChem 2010, 11, 1107–1119, 10.1002/cphc.200901002 |
| 349 Impact of amino acid substitutions near the catalytic site on the spectral properties of an O2-tolerant membrane-bound [NiFe]-hydrogenase M. Saggu, M. Ludwig, B. Friedrich, P. Hildebrandt, R. Bittl, F. Lendzian, O. Lenz, I. Zebger ChemPhysChem 2010, 11, 1215–1224, 10.1002/cphc.200900988 |
| 348 Photosynthetic hydrogen production by a hybrid complex of photosystem I and [NiFe]-hydrogenase H. Krassen, A. Schwarze, B. Friedrich, K. Ataka, O. Lenz, J. Heberle ACS Nano 2009, 3, 4055–4061, 10.1021/nn900748j |
| 347 A kinetic and thermodynamic understanding of O2 tolerance in [NiFe]-hydrogenases J. A. Cracknell, A. F. Wait, O. Lenz, B. Friedrich, F. A. Armstrong Proceedings of the National Academy of Sciences of the United States of America 2009, 106, 20681–20686, 10.1073/pnas.0905959106 |
| 345 Comparison of the membrane-bound [NiFe] hydrogenases from R. eutropha H16 and D. vulgaris Miyazaki F in the oxidized ready state by pulsed EPR M. Saggu, C. Teutloff, M. Ludwig, M. Brecht, M. E. Pandelia, O. Lenz, B. Friedrich, W. Lubitz, P. Hildebrandt, F. Lendzian, R. Bittl Physical Chemistry Chemical Physics 2010, 12, 2139–2148, 10.1039/b922236g |
| 136 Enzymatic oxidation of H2 in atmospheric O2: The electrochemistry of energy generation from trace H2 by aerobic by aerobic microorganisms J. A. Cracknell, K. A. Vincent, M. Ludwig, O. Lenz, B. Friedrich, F. A. Armstrong Journal of the American Chemical Society 2008, 130, 424–425, 10.1021/ja078299 |
| 135 Hydrogen production under aerobic conditions by membrane-bound hydrogenases from Ralstonia species G. Goldet, A. F. Wait, J. A. Cracknell, K. A. Vincent, M. Ludwig, O. Lenz, B. Friedrich, F. A. Armstrong Journal of the American Chemical Society 2008, 130, 11106–11113, 10.1021/ja8027668 |
| 133 Concerted action of two novel auxiliary proteins in assembly of the active site in a membrane-bound [NiFe]-hydrogenase M. Ludwig, T. Schubert, I. Zebger, N. Wisitruangsakul, M. Saggu, A. Strack, O. Lenz, P. Hildebrandt, B. Friedrich The Journal Of Biological Chemistry 2009, 284, 2159–2168, 10.1074/jbc.M808488200 |
| 129 Biokatalysatoren für die Erzeugung von Wasserstoff und Strom O. Lenz, B. Friedrich Aktuelle Wochenschau (Online) 2008, |
| 52 Spectroscopic insight into the oxygen-tolerant membrane-associated [NiFe]-hydrogenase of Ralstonia eutropha H16 M. Saggu, I. Zebger, M. Ludwig, O. Lenz, B. Friedrich, P. Hildebrandt, F. Lendzian Journal of Biological Chemistry 2009, 284, 16264–16276, 10.1074/jbc.M805690200 |
| 49 Untersuchung des Katalyseprozesses der membrangebundenen Hydrogenase aus Rastolnia EutrophaH16 mittels oberflächenverstärkter Infrarotabsorptionsspektroskopie N. Wisitruangsakul, O. Lenz, M. Ludwig, B. Friedrich, P. Hildebrandt, F. Lendzian, I. Zebger Angewandte Chemie 2009, 121, 621–623, 10.1002/ange.200802633 |
| 48 Monitoring catalysis of the membrane-bound hydrogenase of Rastolnia Eutropha by surface enhanced infrared absorption spectroscopy N. Wisitruangsakul, O. Lenz, M. Ludwig, B. Friedrich, P. Hildebrandt, F. Lendzian, I. Zebger Angewandte Chemie International Edition 2009, 48, 611–613, 10.1002/anie.200802633 |