The group of Prof. Budisa could demonstrate that it is possible to introduce three types of non-canonical amino acids into a protein in a single expression experiment.
The use of non-canonical amino acids in protein engineering gained much attention in recent years. Genetic code engineering is based on in vivo sense-codon reassignment, whereby synthetic amino acids are translated into protein sequences in residue-specific manner. In this way proteins are chemically enriched in a controlled manner without need for DNA mutagenesis. But until now, only one type of non-canonical amino acids could be introduced into protein sequences...
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You can find the full article of Nediljko Budisa and coworkers at Research\Research Highlights.